RAD Data comm FLM-1 Guide de l'utilisateur télécharger pdf (Page 290)

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101

Quantity One User Guide

15-14

If you have deﬁned standards in the gel, a pop-up box will warn you that the

regression model for calculating band values will be restricted to point-to-

point semi-log. If you selected a different regression model when deﬁning

standards, it will be changed.

The ﬁrst time you click on an experimental lane with the Match command, a

pop-up box will prompt you to specify the matching tolerance.

Fig. 15-11. Query box: Apply matching to the whole lane?

Note: Tolerance is the minimum spacing that the matching model expects to

ﬁnd between unique bands. It is expressed as a percent of lane height.

You can enter a value between 0.2 and 10 percent. If your bands are very

close together, enter a tolerance of 2.5 percent or less.

After you select a tolerance, click on Yes to automatically match all the bands

in the image. (Click on No to match only the speciﬁc band you clicked on.)

When you click on Yes, the bands in the lane you selected will change to

green, indicating that they are known band types that have been identiﬁed by

you. A band type number will appear next to each band.

The automatic matching mechanism will attempt to match the bands in your

image to the known band types. Matched bands are labeled in red, with the

number of the band type appearing next to each band. These matched bands

are connected by modeling lines.

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RAD Data comm FLM-1 Guide de l'utilisateur Page 290

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